SeePhase™
The SeePhase™ system is a fiber-optic fluorescence lifetime system that uses ROI’s proprietary frequency domain “phase-locked” multi-channel fluorescence lifetime measurement technology suitable for the real time detection, identification, and classification of chemical and biological fluorescent marker activity including auto-fluorescence in biological systems. The SeePhase™ is a frequency-modulation, intensity independent, system that offers the ability to monitor the fluorescence lifetime “fingerprint” signature of target fluorophores in the presence of background auto-fluorescence interference.

Model No. | 500 | 1500 | 5000 | 5300 | 5300-I |
---|---|---|---|---|---|
Monitoring Mode | Frequency vs. Time | Frequency vs. Time | Phase vs. Frequency | Phase vs. Frequency | Phase vs. Frequency |
Frequency Mode | Phase-Locked | Phase-Locked | Frequency Sweep | Frequency Sweep | Frequency Sweep |
Fluorescence Lifetime Range | 140 μsec – 100 nsec | 140 μsec – 100 nsec | 140 μsec – 200 psec | 70 μsec – 50 psec | 70 μsec – 100 psec |
Modulation Frequency Range | 20 kHz to 200 kHz | 20 kHz to 200 kHz | 20 kHz to 100 MHz | 50 kHz to 400 MHz | 20 kHz to 300 MHz |
Excitation Source Type | Light Emitting Diode | Laser Diode | Laser Diode | Laser Diode | Laser Diode |
Excitation Source Wavelength | 250-nm to 850-nm | 360-nm to 850-nm | 360-nm to 850-nm | 360-nm to 850-nm | 360-nm to 850-nm |
Excitation Source Power | 1-mW to 10-mW | 1-mW to 1000-mW | 1-mW to 1000-mW | 1-mW to 1000-mW | 1-mW to 1000-mW |
Detector | APD or PMT | PMT | 2D – PMT | 2D – PMT | CCD Camera |
Emission Wavelength Selection | Optical Filter | Optical Filter | Optical Filter | Optical Filter | Optical Filter |
Remote Sensing | Fiber Bundle | Imaging Telescope | Imaging Telescope | Imaging Telescope | Imaging Telescope |
Laser Scanner | N/A | 2-D Galvanometer | 2-D Galvanometer | 2-D Galvanometer | 2-D Full CCD Imaging |
Fiber Optic Connector | Multimode-SMA | ||||
Data Display | LabView Graphical Interface | ||||
Data Communication | RS-232/USB | ||||
Power Supply | 12V/500 mA | 115/220 VAC External | 115/220 VAC External | 115/220 VAC External | 115/220 VAC External |